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protogel 31303 to calculate the volumes of ProtoGel, ProtoGel Resolving Buffer and other reagents needed. Volumes of Solution Components for Common Gel Percentages mL Using Premixed 4X Resolving Buffer or 1.5M Tris-HCl Gel % -OR-6 ProtoGel 30% 20.0 ProtoGel 30% 20.0 4X Resolving Buffer 25.0 1.5M Tris-HCl pH8.8 25.0 Deionized Water 53.9 10% SDS 1.0

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